info:eu-repo/semantics/article
Label-free graphene oxide–based SPR genosensor for the quantification of microRNA21
Fecha
2020-06Registro en:
López Mujica, Michael Earvin; Zhang, Yuanyuan; Bédioui, Féthi; Gutierrez, Fabiana Andrea; Rivas, Gustavo Adolfo; Label-free graphene oxide–based SPR genosensor for the quantification of microRNA21; Springer Heidelberg; Analytical and Bioanalytical Chemistry; 412; 15; 6-2020; 3539-3546
1618-2642
1618-2650
CONICET Digital
CONICET
Autor
López Mujica, Michael Earvin
Zhang, Yuanyuan
Bédioui, Féthi
Gutierrez, Fabiana Andrea
Rivas, Gustavo Adolfo
Resumen
This work is focused on the development of a genosensor for microRNA-21 quantification using surface plasmon resonance (SPR) to transduce the hybridization event. The biosensing platform was built by self-assembling two bilayers of poly(diallyldimethylammonium chloride) (PDDA) and graphene oxide (GO) at a gold surface modified with 3-mercaptopropane sulfonate (MPS), followed by the covalent attachment of the DNA probe. GO was used in two directions, to allow the anchoring of the probe DNA and to increase the sensitivity of the biosensing event due to its field enhancer effect. The new bioanalytical platform represents an interesting alternative for the label-free biosensing of microRNA-21, with a linear range between 1.0 fM and 10 nM, a sensitivity of 5.1 ± 0.1 moM−1 and a detection limit of 0.3fM. The proposed sensing strategy was successfully used for the quantification of microRNA-21 in enriched urine samples.