info:eu-repo/semantics/article
Interaction of the small-molecule kinase inhibitors tofacitinib and lapatinib with membranes
Fecha
2020-11Registro en:
Haralampiev, Ivan; Alonso de Armiño, Diego Javier; Luck, Meike; Fischer, Markus; Abel, Tobias; et al.; Interaction of the small-molecule kinase inhibitors tofacitinib and lapatinib with membranes; Elsevier Science; Biochimica et Biophysica Acta - Biomembranes; 1862; 183414; 11-2020; 1-12
0005-2736
CONICET Digital
CONICET
Autor
Haralampiev, Ivan
Alonso de Armiño, Diego Javier
Luck, Meike
Fischer, Markus
Abel, Tobias
Huster, Daniel
Di Lella, Santiago
Scheidt, Holger A.
Müller, Peter
Resumen
Lapatinib and tofacitinib are small-molecule kinase inhibitors approved for the treatment of advanced or metastatic breast cancer and rheumatoid arthritis, respectively. So far, the mechanisms which are responsible for their activities are not entirely understood. Here, we focus on the interaction of these drug molecules with phospholipid membranes, which has not yet been investigated before in molecular detail. Owing to their lipophilic characteristics, quantitatively reflected by large differences of the partition equilibrium between water and octanol phases (expressed by logP values), rather drastic differences in the membrane interaction of both molecules have to be expected. Applying experimental (nuclear magnetic resonance, fluorescence and ESR spectroscopy) and theoretical (molecular dynamics simulations) approaches, we found that lapatinib and tofacitinib bind to lipid membranes and insert into the lipid-water interface of the bilayer. For lapatinib, a deeper embedding into the membrane bilayer was observed than for tofacitinib implying different impacts of the molecules on the bilayer structure. While for tofacitinib, no influence to the membrane structure was found, lapatinib causes a membrane disturbance, as concluded from an increased permeability of the membrane for polar molecules. These data may contribute to a better understanding of the cellular uptake mechanism(s) and the side effects of the drugs.