info:eu-repo/semantics/article
Evaluation of biomarkers in glioma by immunohistochemistry on paraffin- embedded 3D glioma neurosphere cultures
Fecha
2019-01-09Registro en:
Núñez Aguilera, Felipe Javier; Mendez, Flor M.; Garcia Fabbiani, María B.; Pardo, Joaquin; Edwards, Marta; et al.; Evaluation of biomarkers in glioma by immunohistochemistry on paraffin- embedded 3D glioma neurosphere cultures; Journal of Visualized Experiments; Journal of Visualized Experiments; 2019; 143; 9-1-2019; 1-7
1940-087X
CONICET Digital
CONICET
Autor
Núñez Aguilera, Felipe Javier
Mendez, Flor M.
Garcia Fabbiani, María B.
Pardo, Joaquin
Edwards, Marta
Lowenstein, Pedro R.
Castro, María G.
Resumen
Analysis of protein expression in glioma is relevant for several aspects in the study of its pathology. Numerous proteins have been described asbiomarkers with applications in diagnosis, prognosis, classification, state of tumor progression, and cell differentiation state. These analyses ofbiomarkers are also useful to characterize tumor neurospheres (NS) generated from glioma patients and glioma models. Tumor NS provide avaluable in vitro model to assess different features of the tumor from which they are derived and can more accurately mirror glioma biology. Herewe describe a detailed method to analyze biomarkers in tumor NS using immunohistochemistry (IHC) on paraffin-embedded tumor NS.