info:eu-repo/semantics/article
Composite cellulose fibre for affinity chromatography application
Fecha
2019-04Registro en:
Carbajal, Maria Laura; Kikot, Pamela Alejandra; Torchio, Gabriela María; Fernandez Lahore, M.; Grasselli, Mariano; Composite cellulose fibre for affinity chromatography application; Editura Acad Romane; Cellulose Chemistry And Technology; 54; 1-2; 4-2019; 125-134
0576-9787
CONICET Digital
CONICET
Autor
Carbajal, Maria Laura
Kikot, Pamela Alejandra
Torchio, Gabriela María
Fernandez Lahore, M.
Grasselli, Mariano
Resumen
Affinity chromatographic supports is nowadays one of the most used and expensive consumable material for protein purification at the laboratory and industrial scale. The introduction of cost-effective materials is an important issue to address to spread the usefulness of this technology. Cotton fibres are a highly available natural material with excellent mechanical and structural properties which can be used for this propose. Nevertheless, fibre insolubility and low chemical reactivity are the major drawbacks to use this material for protein chromatography.In this work, a composite material prepared from polymethacrylate/cellulose fibres were used for the preparation of chromatographic materials containing immobilised proteins as adsorptive ligands. A green fluorescent protein and a protein A simil ligand containing cysteine in the terminal tags were immobilised onto the fibres by the epoxy-thiol chemistry. Buffer salt, pH, reaction time and pre-swelling procedure were optimised. Materials were characterised by fluorescence and electron microscopes, in addition to the binding capacity of the adsorbent materials.