info:eu-repo/semantics/article
Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species
Fecha
2019-10Registro en:
Chabaud, Mireille; Fournier, Joëlle; Brichet, Lukas; Abdou Pavy, Iltaf; Imanishi, Leandro Ezequiel; et al.; Chitotetraose activates the fungal-dependent endosymbiotic signaling pathway in actinorhizal plant species; Public Library of Science; Plos One; 14; 10; 10-2019; 1-13; e0223149
1932-6203
CONICET Digital
CONICET
Autor
Chabaud, Mireille
Fournier, Joëlle
Brichet, Lukas
Abdou Pavy, Iltaf
Imanishi, Leandro Ezequiel
Brottier, Laurent
Pirolles, Elodie
Hocher, Valérie
Franche, Claudine
Bogusz, Didier
Wall, Luis Gabriel
Svistoonoff, Sergio
Gherbi, Hassen
Barker, David G.
Resumen
Mutualistic plant-microbe associations are widespread in natural ecosystems and have made major contributions throughout the evolutionary history of terrestrial plants. Amongst the most remarkable of these are the so-called root endosymbioses, resulting from the intracellular colonization of host tissues by either arbuscular mycorrhizal (AM) fungi or nitrogen-fixing bacteria that both provide key nutrients to the host in exchange for energy-rich photosynthates. Actinorhizal host plants, members of the Eurosid 1 clade, are able to associate with both AM fungi and nitrogen-fixing actinomycetes known as Frankia. Currently, little is known about the molecular signaling that allows these plants to recognize their fungal and bacterial partners. In this article, we describe the use of an in vivo Ca2+ reporter to identify symbiotic signaling responses to AM fungi in roots of both Casuarina glauca and Discaria trinervis, actinorhizal species with contrasting modes of Frankia colonization. This approach has revealed that, for both actinorhizal hosts, the short-chain chitin oligomer chitotetraose is able to mimic AM fungal exudates in activating the conserved symbiosis signaling pathway (CSSP) in epidermal root cells targeted by AM fungi. These results mirror findings in other AM host plants including legumes and the monocot rice. In addition, we show that chitotetraose is a more efficient elicitor of CSSP activation compared to AM fungal lipo-chitooligosaccharides. These findings reinforce the likely role of short-chain chitin oligomers during the initial stages of the AM association, and are discussed in relation to both our current knowledge about molecular signaling during Frankia recognition as well as the different microsymbiont root colonization mechanisms employed by actinorhizal hosts.