info:eu-repo/semantics/article
Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation
Fecha
2019-07-30Registro en:
Piñuel, Maria Lucrecia; Boeri, Patricia; Zubillaga, Fanny; Barrio, Daniel Alejandro; Torreta, Joaquin; et al.; Production of white, red and black quinoa (Chenopodium quinoa willd var. real) protein isolates and its hydrolysates in germinated and non-germinated quinoa samples and antioxidant activity evaluation; Multidisciplinary Digital Publishing Institute; Plants; 8; 8; 30-7-2019; 1-18
2223-7747
CONICET Digital
CONICET
Autor
Piñuel, Maria Lucrecia
Boeri, Patricia
Zubillaga, Fanny
Barrio, Daniel Alejandro
Torreta, Joaquin
Cruz, Andrea
Vásquez, Grace
Pinto, Adelita
Carrillo, Wilman
Resumen
Red, black and white seeds quinoa were germinated at 28◦ C during 24 (G1), 48 and 72 h (G3). Red quinoa presented a higher percentage of germination with a value of 46% of germination at 72 h. Quinoa protein isolate (QPI) was obtained by alkaline extraction (pH 8.0) followed by an isoelectric precipitation (pH 4.5) from white, red and black quinoa seeds, germinated QPI-G1 or QPI-G3 and non-germinated QPI-NG, Chenopodium quinoa Willd var. Real. QPI-G1, QPI-G3 and QPI-NG were subject to a simulated gastric digestion (DG) and in vitro duodenal digestion (DD). The antioxidant activity was evaluated using the 1, 1-diphenyl-2-picryl hydrazyl (DPPH), azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and oxygen radical absorbance capacity (ORAC) methods. Gastric and duodenal digest of QPI-NG and QPI-G1 and QPI-G3 from white, red and black quinoa presented antioxidant activity. QPI-G1-DD of white quinoa presented the highest antioxidant activity with a DPPH value of 167.98 µmoL TE/g of digest, QPI-G1-DD of red quinoa with an ABTS value of 204.86 µmoL TE/g of digest and QPI-G1-DD of black quinoa with an ORAC value of 401.42 µmoL TE/g of digest. QPI-G3-DD of white quinoa presented higher antioxidant activity with a DPPH value of 186.28 µmoL TE/g of sample, QPI-G3-DD of red quinoa with an ABTS value of 144.06 µmoL TE/g of digest and QPI-G3-DD of black quinoa with an ORAC value of 395.14 µmoL TE/g of digest. The inhibition of reactive oxygen species (ROS) production in the zebrafish embryo model (Danio rerio) was evaluated. Protein profiles of QPI from white, red and black from germinated quinoa and non-germinated quinoa were similar with proteins between 10 kDa to 100 kDa with the presence of globulins 11S and 7S and 2S albumins.
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