info:eu-repo/semantics/article
Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats
Fecha
2014-02Registro en:
Dealtry, Simone; Ding, Guo Chun; Weichelt, Viola; Dunon, Vincent; Andreas, Schlüter; et al.; Cultivation-independent screening revealed hot spots of IncP-1, IncP-7 and IncP-9 plasmid occurrence in different environmental habitats; Public Library of Science; Plos One; 9; 2-2014; 9-15
1932-6203
CONICET Digital
CONICET
Autor
Dealtry, Simone
Ding, Guo Chun
Weichelt, Viola
Dunon, Vincent
Andreas, Schlüter
Martini, María Carla
del Papa, Maria Florencia
Lagares, Antonio
Auton Amos, Gregory Charles
Wellington, Elizabeth Margaret Helen
Gaze, William Hugo
Sipkema, Detmer
Sjoiling, Sara
Springael, Dirk
Heuer, Holger
van Elsas, Jan Dirk
Thomas, Christopher
Smalla, Kornelia
Resumen
IncP-1, IncP-7 and IncP-9 plasmids often carry genes encoding enzymes involved in the degradation of man-made and natural contaminants, thus contributing to bacterial survival in polluted environments. However, the lack of suitable molecular tools often limits the detection of these plasmids in the environment. In this study, PCR followed by Southern blot hybridization detected the presence of plasmid-specific sequences in total community (TC-) DNA or fosmid DNA from samples originating from different environments and geographic regions. A novel primer system targeting IncP-9 plasmids was developed and applied along with established primers for IncP-1 and IncP-7. Screening TC-DNA from biopurification systems (BPS) which are used on farms for the purification of pesticide-contaminated water revealed high abundances of IncP-1 plasmids belonging to different subgroups as well as IncP-7 and IncP-9. The novel IncP-9 primer-system targeting the rep gene of nine IncP-9 subgroups allowed the detection of a high diversity of IncP-9 plasmid specific sequences in environments with different sources of pollution. Thus polluted sites are ??hot spots?? of plasmids potentially carrying catabolic genes.