info:eu-repo/semantics/article
Physical mapping of the 5S and 18S-25S rRNA genes by FISH as evidence that Arachis duranensis and A. ipaensis are the wild diploid progenitors of A. hypogaea (Leguminosae)
Fecha
2004-09Registro en:
Seijo, José Guillermo; Lavia, Graciela Inés; Fernandez, Aveliano; Krapovickas, Antonio; Ducasse, Daniel Adrián; et al.; Physical mapping of the 5S and 18S-25S rRNA genes by FISH as evidence that Arachis duranensis and A. ipaensis are the wild diploid progenitors of A. hypogaea (Leguminosae); Botanical Society of America; American Journal of Botany; 91; 9; 9-2004; 1294-1303
0002-9122
1537-2197
CONICET Digital
CONICET
Autor
Seijo, José Guillermo
Lavia, Graciela Inés
Fernandez, Aveliano
Krapovickas, Antonio
Ducasse, Daniel Adrián
Moscone, Eduardo Alberto
Resumen
The 5S and the 18S-25S rRNA genes were physically mapped by fluorescent in situ hybridization (FISH) in all botanical varieties of cultivated peanut Arachis hypogaea (2n = 4x = 40), in the wild tetraploid A. monticola, and in seven wild diploid species considered as putative ancestors of the tetraploids. A detailed karyotype analysis including the FISH signals and the heterochromatic bands was carried out. Molecular cytogenetic landmarks are provided for the construction of a FISH-based karyotype in Arachis species. The size, number, and chromosome position of FISH signals and heterochromatic bands are similar in all A. hypogaea varieties and A. monticola, but vary among the diploid species. Genome constitution of the species is discussed and several chromosome homeologies are established. The bulk of the chromosome markers mapped, together with data on geographical distribution of the taxa, suggest that peanut originated upon domestication of A. monticola and evidence that the diploids A. duranensis and A. ipaensis are the most probable ancestors of both tetraploid species. Allopolyploidy could have arisen by a single event or, if by multiple events, always from the same diploid species.