info:eu-repo/semantics/article
Lysine transporters in human trypanosomatid pathogens
Fecha
2012-01Registro en:
Inbar, Ehud; Canepa, Gaspar Exequiel; Carrillo, Carolina; Glaser, Fabian; Grotemeyer, Marianne Suter; et al.; Lysine transporters in human trypanosomatid pathogens; Springer Wien; Amino Acids; 42; 1; 1-2012; 347-360
0939-4451
1438-2199
CONICET Digital
CONICET
Autor
Inbar, Ehud
Canepa, Gaspar Exequiel
Carrillo, Carolina
Glaser, Fabian
Grotemeyer, Marianne Suter
Rentsch, Doris
Zilberstein, Dan
Pereira, Claudio Alejandro
Resumen
In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively.