info:eu-repo/semantics/article
Crosstalk between sterol and neutral lipid metabolism in the alga Haematococcus pluvialis exposed to light stress
Fecha
2020-10Registro en:
Scodelaro Bilbao, Paola Gabriela; Garelli, Andres; Díaz, Marina Lucía; Salvador, Gabriela Alejandra; Leonardi, Patricia Ines; Crosstalk between sterol and neutral lipid metabolism in the alga Haematococcus pluvialis exposed to light stress; Elsevier Science; Biochimica Et Biophysica Acta - Molecular and Cell Biology of Lipids; 1865; 10; 10-2020; 1-13; 158767
1388-1981
CONICET Digital
CONICET
Autor
Scodelaro Bilbao, Paola Gabriela
Garelli, Andres
Díaz, Marina Lucía
Salvador, Gabriela Alejandra
Leonardi, Patricia Ines
Resumen
The presence, biosynthesis and functional role of sterols in the green microalga Haematococcus pluvialis remain poorly understood. In this work we studied the effect of high-light (HL) stress on sterol synthesis in H. pluvialis UTEX 2505 cells. HL stress induced the synthesis of sterols in parallel with that of triacylglycerides (TAG), giving rise to the synthesis of cholesterol over that of phytosterols. Blockage of the carotenogenic 1-deoxy-D-xylulose 5-phosphate (MEP) pathway is shown to be involved in HL-induced sterol synthesis. In addition, high irradiance exposure induced MEP- and fatty acid (FA)-biosynthetic transcripts. The pharmacological inhibition of these pathways suggests a possible feedback regulation of sterol and FA homeostasis. Finally, both lipid classes proved crucial to the adequate photosynthetic performance of H. pluvialis grown under HL intensity stress. Our findings reveal new insights into H. pluvialis lipid metabolism that contribute to the development of value-added bioproducts from microalgae.