info:eu-repo/semantics/article
Determination of antibacterial activity of film coatings against four clinically relevant bacterial strains
Fecha
2021-01Registro en:
Scilletta, Natalia Antonela; Pezzoni, Magdalena; Desimone, Martín Federico; Soler Illia, Galo Juan de Avila Arturo; Bellino, Martin Gonzalo; et al.; Determination of antibacterial activity of film coatings against four clinically relevant bacterial strains; Bio-protocol; Bio-protocol; 11; 2; 1-2021; 1-11
2331-8325
CONICET Digital
CONICET
Autor
Scilletta, Natalia Antonela
Pezzoni, Magdalena
Desimone, Martín Federico
Soler Illia, Galo Juan de Avila Arturo
Bellino, Martin Gonzalo
Catalano, Paolo Nicolás
Resumen
Antibacterial coatings have currently gained great importance in biomedical technology investigations. Because of the spatial arrangement of the film coatings, evaluation of antibacterial activity presents a new challenge regarding traditional bacterial counting methods. In this protocol, four clinically relevant pathogens, Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus were incubated on titania mesostructured thin film coatings for 24 h. Then, cell viability was studied considering three methods: counting of the number of colony forming units (CFU), live/dead staining, and quantification of extracellular DNA in suspension. Firstly, bacterial count was determined by the standard plate-count technique. Secondly, bacteria membrane integrity was evaluated by utilization of two fluorescent dyes, which allow distinction between live (membrane intact) and dead (membrane disrupted) bacteria. Lastly, extracellular DNA was quantified by spectrophotometry. In this manner, the three aforementioned techniques enabled the study of bacterial viability by qualitative and quantitative analyses.