info:eu-repo/semantics/article
Improved hepatic MRP2/ABCC2 transport activity in LPS-induced cholestasis by aquaporin-1 gene transfer
Fecha
2019-10Registro en:
Marrone, Julieta; Tocchetti, Guillermo Nicolás; Danielli, Mauro; Mottino, Aldo Domingo; Marinelli, Raul Alberto; Improved hepatic MRP2/ABCC2 transport activity in LPS-induced cholestasis by aquaporin-1 gene transfer; Elsevier France-editions Scientifiques Medicales Elsevier; Biochimie; 165; 10-2019; 179-182
0300-9084
CONICET Digital
CONICET
Autor
Marrone, Julieta
Tocchetti, Guillermo Nicolás
Danielli, Mauro
Mottino, Aldo Domingo
Marinelli, Raul Alberto
Resumen
Multidrug resistance-associated protein 2 (MRP2/ABCC2), a hepatocyte canalicular transporter involved in bile secretion, is downregulated in cholestasis triggered by lipopolysaccharide. The human aquaporin-1 (hAQP1) adenovirus-mediated gene transfer to liver improves cholestasis by incompletely defined mechanisms. Here we found that hAQP1 did not affect MRP2/ABCC2 expression, but significantly increased its transport activity assessed in situ with endogenous and exogenous substrates, likely by a hAQP1-induced increase in canalicular membrane cholesterol amount. Our results suggest that hAQP1-induced MRP2/ABCC2 activation contributes to the cholestasis improvement.