info:eu-repo/semantics/article
Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry
Fecha
2018-05Registro en:
Figueroa Espinosa, Roque Arnulfo; Rumi, María Valeria; Marchisio, Martin Luis; Cejas, Daniela; Radice, Marcela Alejandra; et al.; Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry; Elsevier Science; Journal of Microbiological Methods; 148; 5-2018; 22-28
0167-7012
CONICET Digital
CONICET
Autor
Figueroa Espinosa, Roque Arnulfo
Rumi, María Valeria
Marchisio, Martin Luis
Cejas, Daniela
Radice, Marcela Alejandra
Vay, Carlos Alberto
Barrios, Rubén
Gutkind, Gabriel Osvaldo
Di Conza, José Alejandro
Resumen
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000–45,000 Da showed that two groups of isolates could be differentiated after peak analysis. A single distinctive peak with different intensities, at approximately m/z 39,800 Da was found in all CMY-2 producing strains (transconjugant, transformant and wild type) and consistently absent in the control groups (ESBL producers and susceptible strains). Statistical results showed 100% values for sensitivity and specificity, indicating a perfect test and a high discriminative power. In this study, we demonstrated that MALDI-TOF MS has the potential to detect directly the most clinically relevant acquired AmpC β-lactamase, the CMY-2-enzyme, in E. coli with a less time-consuming process as compared to conventional methods. Our results may constitute the basis for further research to detect other β-lactamases, or even other resistance markers.