info:eu-repo/semantics/article
Determination of cobalt in urine by FI-ICP-AES with online preconcentration
Fecha
2002-09-01Registro en:
Farias, Gustavo Marcelo; Wuilloud, Rodolfo German; Moyano, Susana; Gasquez, José Antonio; Olsina, Roberto Antonio; et al.; Determination of cobalt in urine by FI-ICP-AES with online preconcentration; Preston Publ Inc; Journal Of Analytical Toxicology; 26; 6; 1-9-2002; 360-364
0146-4760
1945-2403
CONICET Digital
CONICET
Autor
Farias, Gustavo Marcelo
Wuilloud, Rodolfo German
Moyano, Susana
Gasquez, José Antonio
Olsina, Roberto Antonio
Martinez, Luis Dante
Resumen
A method for the preconcentration and determination of cobalt in human urine samples was developed. The online preconcentration and determination were attained using inductively coupled plasma atomic emission spectromety (ICP-AES) coupled to a flow injection (FI) method. Cobalt was retained on an Amberlite XAD-7 resin as cobalt-2-(5-bromo-2-pyridylazo)-5-diethylaminophenol complex at pH 9.5. Cobalt was removed from the microcolumn with perchloric acid. A sensitivity enhancement factor of 90 was obtained with respect to cobalt determination by ICP-AES without preconcentration. The value of detection limit for the preconcentration method proposed was 25 ng/L. The precision for 10 replicate determinations at the 5 mg/L (mean ± SD, 5.1 ± 0.14) Co level was 2.7% relative standard deviation, calculated from the peak heights obtained. The calibration graph preconcentration method for cobalt was linear with a correlation coefficient of 0.9994 from approximately 0.25 mg/L up to at least 100 mg/L. The method was successfully applied to the determination of cobalt in human urine samples.