info:eu-repo/semantics/article
Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression
Fecha
2005-03Registro en:
Appelbaum, Lior; Anzulovich Miranda, Ana Cecilia; Baler, Ruben; Gothilf, Yoav; Homeobox-Clock protein interaction in zebrafish: a shared mechanism for pineal-specific and circadian gene expression; American Society for Biochemistry and Molecular Biology; Journal of Biological Chemistry; 280; 12; 3-2005; 11544-11551
0021-9258
CONICET Digital
CONICET
Autor
Appelbaum, Lior
Anzulovich Miranda, Ana Cecilia
Baler, Ruben
Gothilf, Yoav
Resumen
In non-mammalian vertebrates, the pineal gland is photoreceptive and contains an intrinsic circadian oscillator that drives rhythmic production and secretion of melatonin. These features require an accurate spatiotemporal expression of an array of specific genes in the pineal gland. Among these is the arylalkylamine N-acetyltransferase, a key enzyme in the melatonin production pathway. In zebrafish, pineal specificity of zfaanat2 is determined by a region designated the pineal-restrictive downstream module (PRDM), which contains three photoreceptor conserved elements (PCEs) and an E-box, elements that are generally associated with photoreceptor-specific and rhythmic expression, respectively. Here, by using in vivo and in vitro approaches, it was found that the PCEs and E-box of the PRDM mediate a synergistic effect of the photoreceptor-specific homeobox OTX5 and rhythmically expressed clock protein heterodimer, BMAL/CLOCK, on zfaanat2 expression. Furthermore, the distance between the PCEs and the E-box was found to be critical for PRDM function, suggesting a possible physical feature of this synergistic interaction. OTX5-BMAL/CLOCK may act through this mechanism to simultaneously control pineal-specific and rhythmic expression of zfaanat2 and possibly also other pineal and retinal genes.