info:eu-repo/semantics/article
Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae)
Fecha
2021-06Registro en:
Schneider, Rosio Gabriela; Ferro, Juan Martín; Reinko, Ivana Noelia; Boeris, Juan Martín; Cardozo, Dario Elbio; et al.; Sex chromosomes in the Vizcacheras’ White-lipped frog, Leptodactylus bufonius (Anura, Leptodactylidae); Academia Brasilera de Ciencias; Anais da Academia Brasileira de Ciências; 93; 2; 6-2021; 1-12
0001-3765
1678-2690
CONICET Digital
CONICET
Autor
Schneider, Rosio Gabriela
Ferro, Juan Martín
Reinko, Ivana Noelia
Boeris, Juan Martín
Cardozo, Dario Elbio
Baldo, Juan Diego
Resumen
Cytogenetic analyses were performed on specimens of Leptodactylus bufonius from different localities in Argentina. Mitotic chromosomes were studied with Giemsa and differential staining techniques (Ag-NOR, C-banding, and CMA3 /DAPI) and fl uorescence in situ hybridization with the 18S DNAr probe. All specimens showed karyotypes with 2n = 2x = 22 and FN = 44. Secondary constrictions were present in the long arm of chromosome pair 8, coincident with Ag-NOR and hybridization signals of the 18S DNAr probe. The C-banding technique evidenced an important amount of heterochromatin with a sex-linked pericentromeric band in the short arm of chromosome pair 4. This heterochromatic band was heteromorphic in males but present in both homologues of females, and it was CMA3 positive (DAPI negative) at fl uorescence staining. The occurrence of heteromorphic XY sex chromosomes in L. bufonius is the second known case in Leptodactylus and the fi fth within the speciose family Leptodactylidae.