dc.creator | Kolmakov, Kirill | |
dc.creator | Wurm, Christian | |
dc.creator | Sednev, Maksim V. | |
dc.creator | Bossi, Mariano Luis | |
dc.creator | Belov, Vladimir N. | |
dc.creator | Hell, Stefan W. | |
dc.date.accessioned | 2019-02-26T19:04:29Z | |
dc.date.accessioned | 2022-10-15T01:43:37Z | |
dc.date.available | 2019-02-26T19:04:29Z | |
dc.date.available | 2022-10-15T01:43:37Z | |
dc.date.created | 2019-02-26T19:04:29Z | |
dc.date.issued | 2012-03 | |
dc.identifier | Kolmakov, Kirill; Wurm, Christian; Sednev, Maksim V.; Bossi, Mariano Luis; Belov, Vladimir N.; et al.; Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group; Royal Society of Chemistry; Photochemical and Photobiological Sciences; 11; 3; 3-2012; 522-532 | |
dc.identifier | 1474-905X | |
dc.identifier | http://hdl.handle.net/11336/70877 | |
dc.identifier | CONICET Digital | |
dc.identifier | CONICET | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4331329 | |
dc.description.abstract | Caged near-IR emitting fluorescent dyes are in high demand in optical microscopy but up to now were unavailable. We discovered that the combination of a carbopyronine dye core and a photosensitive 2-diazo-1-indanone residue leads to masked near-IR emitting fluorescent dyes. Illumination of these caged dyes with either UV or visible light (λ < 420 nm) efficiently generates fluorescent compounds with absorption and emission at 635 nm and 660 nm, respectively. A high-yielding synthetic route with attractive possibilities for further dye design is described in detail. Good photostability, high contrast, and a large fluorescence quantum yield after uncaging are the most important features of the new compounds for non-invasive imaging in high-resolution optical microscopy. For use in immunolabelling the caged dyes were decorated with a (hydrophilic) linker and an (activated) carboxyl group. | |
dc.language | eng | |
dc.publisher | Royal Society of Chemistry | |
dc.relation | info:eu-repo/semantics/altIdentifier/url/http://pubs.rsc.org/en/content/articlelanding/2012/pp/c1pp05321c/unauth | |
dc.relation | info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1039/C1PP05321C | |
dc.rights | https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | Cage Compounds | |
dc.subject | Superresolution Microscopy | |
dc.subject | Carbopyronines Carbopyronines | |
dc.title | Masked red-emitting carbopyronine dyes with photosensitive 2-diazo-1-indanone caging group | |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:ar-repo/semantics/artículo | |
dc.type | info:eu-repo/semantics/publishedVersion | |