info:eu-repo/semantics/article
Direct binding of benzoate derivatives to two chemoreceptors with Cache sensor domains in Halomonas titanicae KHS3
Fecha
2020-10-24Registro en:
Gasperotti, Ana Florencia; Herrera Seitz, Karina; Balmaceda, Rocio Soledad; Prosa, Luciano M.; Jung, Kirsten; et al.; Direct binding of benzoate derivatives to two chemoreceptors with Cache sensor domains in Halomonas titanicae KHS3; Wiley Blackwell Publishing, Inc; Molecular Microbiology; 115; 4; 24-10-2020; 672-683
0950-382X
CONICET Digital
CONICET
Autor
Gasperotti, Ana Florencia
Herrera Seitz, Karina
Balmaceda, Rocio Soledad
Prosa, Luciano M.
Jung, Kirsten
Studdert, Claudia Alicia
Resumen
Halomonas titanicae KHS3, isolated from a hydrocarbon-contaminated sea harbor in Argentina, is able to grow on aromatic hydrocarbons and displays chemotaxis toward those compounds. This behavior might contribute to the efficiency of its degradation capacity. Using high throughput screening, we identified two chemoreceptors (Htc1 and Htc2) that bind benzoate derivatives and other organic acids. Whereas Htc1 has a high affinity for benzoate (Kd 112 µM) and 2-hydroxybenzoate (Kd 83 µM), Htc2 binds 2-hydroxybenzoate with low affinity (Kd 3.25 mM), and also C3/C4 dicarboxylates. Both chemoreceptors are able to trigger a chemotactic response of E. coli cells to the specific ligands. A H. titanicae htc1 mutant has reduced chemotaxis toward benzoate, and is complemented upon expression of the corresponding receptor. Both chemoreceptors have a Cache-type sensor domain, double (Htc1) or single (Htc2), and their ability to bind aromatic compounds is reported here for the first time.