Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusks

Abstract

Electronic paramagnetic resonance (EPR) is an appropriate tool to identify free radicals formed in tissues under normal as well as stressful conditions. Since nitric oxide (NO) as a free radical has paramagnetic properties it can be detected by EPR. The use of spin traps highly improves the sensitivity allowing NO identification, detection and quantification at room temperature in vitro and in vivo conditions. NO production in animals is almost exclusively associated to an enzyme family known as Nitric Oxide Synthases (NOSs). The digestive glands of mollusks are a major target for oxidative disruption related to environmental stress. A simple EPR-methodology to asses both, the presence of NO and its rate of generation in tissues from different mollusk species, is reported here.