info:eu-repo/semantics/article
DNA damage exerted by mixtures of commercial formulations of glyphosate and imazethapyr herbicides in Rhinella arenarum (Anura, Bufonidae) tadpoles
Fecha
2019-03Registro en:
Carvalho, Wanessa F. ; Ruiz de Arcaute, Celeste; Pérez Iglesias, Juan Manuel; Laborde, Milagros Rosa Raquel; Soloneski, Sonia Maria Elsa; et al.; DNA damage exerted by mixtures of commercial formulations of glyphosate and imazethapyr herbicides in Rhinella arenarum (Anura, Bufonidae) tadpoles; Springer; Ecotoxicology; 28; 3; 3-2019; 367-377
0963-9292
CONICET Digital
CONICET
Autor
Carvalho, Wanessa F.
Ruiz de Arcaute, Celeste
Pérez Iglesias, Juan Manuel
Laborde, Milagros Rosa Raquel
Soloneski, Sonia Maria Elsa
Larramendy, Marcelo Luis
Resumen
Glyphosate (GLY) and imazethapyr (IMZT) are two herbicides commonly used worldwide, either alone or in mixtures. They represent key pesticides in modern agricultural management. The toxicity that results when employed as mixtures has not been characterized so far. Acute toxicity of the 48% GLY-based herbicide (GBH) Credit® and the 10.59% IMZT-based herbicide (IBH) Pivot® H alone and their binary combinations was analyzed in Rhinella arenarum tadpoles exposed in a semi-static renewal test. Lethal effects were determined using mortality as the end-point, whereas sublethal effects were determined employing the single-cell gel electrophoresis (SCGE) bioassay. Based on mortality experiments, results revealed LC5096 h values of 78.18 mg/L GBH and 0.99 mg/L IBH for Credit® and Pivot® H, respectively. An increase in the genetic damage index (GDI) was found after exposure to Credit® or Pivot® H at 5 and 10% of LC5096 h values. The combinations of 5% Credit®-5% Pivot® H LC5096 h and 10% Credit®-10% Pivot® H LC5096 h concentrations significantly enhanced the GDI in comparison with tadpoles exposed only to Credit® or Pivot® H. Thus, the effect of interaction between GBH and IBH inducing DNA damage in R. arenarum blood cells can be considered to be synergistic.