info:eu-repo/semantics/article
Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina
Fecha
2018-12Registro en:
Duo Saito, Rubí Azul; Connell, L.; Rodriguez, R.; Redman, Damian Patricio; Libkind Frati, Diego; et al.; Metabarcoding analysis of the fungal biodiversity associated with Castaño Overa Glacier – Mount Tronador, Patagonia, Argentina; Elsevier; Fungal Ecology; 36; 12-2018; 8-16
1754-5048
CONICET Digital
CONICET
Autor
Duo Saito, Rubí Azul
Connell, L.
Rodriguez, R.
Redman, Damian Patricio
Libkind Frati, Diego
de Garcia, Virginia
Resumen
Cold environments represent the largest fraction of Earth's biosphere, and are known habitats for cold-adapted microorganisms. The aim of this study was to assess the occurrence and biodiversity of cold-adapted fungi associated with the Castaño Overo glacier, Mount Tronador, Patagonia, Argentina. Samples of naked soil, glacial ice and snow were collected and analyzed using tag-encoded 454 pyrosequencing of the nuclear ribosomal internal transcribed spacer (ITS). A total of 1082 OTUs (operational taxonomic units) and 151,669 sequences were obtained. OTUs obtained from soil samples corresponded mainly to the phylum Ascomycota, whereas for snow and ice samples the phylum Basidiomycota was the most represented group. Metabarcoding analysis showed high biodiversity in glacial environments and allowed the detection of hitherto unknown taxa for Patagonia. To the best of our knowledge, this is the first report of fungal diversity in an extreme glacial environment of Patagonian Argentina using amplicon sequencing.