info:eu-repo/semantics/publishedVersion
Evaluation of the immunogenicity of the recombinant protein GST MU of Fasciola hepatica (rFHGSTMu) adsorbed on aluminum hydroxide in sheep
Fecha
2019Registro en:
Evaluation of the immunogenicity of the recombinant protein GST MU of Fasciola hepatica (rFHGSTMu) adsorbed on aluminum hydroxide in sheep; LXIV Reunión Anual de la Sociedad Argentina de Investigación Clínica; LI Reunión Anual de la Asociación Argentina de Farmacología Experimental; XXI Reunión Anual de la Sociedad Argentina de Biología; XXXI Reunión Anual de la Sociedad Argentina de Protozoología; IX Reunión Anual de la Asociación Argentina de Nanomedicinas y VI Reunión Científica Regional de la Asociación Argentina de Ciencia y Tecnología de Animales de Laboratorio; Mar del Plata; Argentina; 2019; 233-233
0025-7680
CONICET Digital
CONICET
Autor
Fernandez, Vanesa
Sola, Javier
Moran, María Celeste
Gutiérrez, Silvina Elena
Estein, Silvia Marcela
Resumen
Fasciola hepatica is a zoonosis which causes significant economic losses in ruminants. The development of a vaccine emerges as an alternative for the control of this disease. The recombinant protein GST Mu from F. hepatica (rFhGSTMu) adsorbed on aluminum hydroxide (rGSTMu + Al(OH)3) conferred 90 % protection against this parasite in the mouse model. The objective of this work was to evaluate the humoral and cellular immune response against this vaccine in the susceptible species. Corriedale female sheeps were immunized twice every 30 days. The humoral immune response was analyzed by an indirect ELISA. Blood was obtained every 15 days from day 0 to 75. The in vivo immune cell response was evaluated on the day 75 by performing the intradermal test with rFhGSTMu. Specific serum IgG antibodies increased after each boost unlike that observed in the control group (without immunization), which maintained baseline levels throughout the trial. The differences were maximum and significative (p<0.05) two weeks after the last immunization. The presence of a cellular immune response was not detected in vivo. This preliminary result indicates that rGSTMu + Al(OH)3 is immunogenic in sheep.