Production of recombinant trypanosoma cruzi antigens in Leishmania tarentolae
Ferrer, María José; Wehrendt, Diana Patricia; Bonilla, Mariana; Comini, Marcelo Alberto; Tellez Iñón, María Teresa; et al.; Production of recombinant trypanosoma cruzi antigens in Leishmania tarentolae; Springer; Methods in Molecular Biology; 1955; 3-2019; 105-118
Ferrer, María José
Wehrendt, Diana Patricia
Comini, Marcelo Alberto
Tellez Iñón, María Teresa
Trypanosomatids are unicellular organisms that colonize a wide diversity of environments and hosts. For instance, Trypanosoma cruzi is a human pathogen responsible for Chagas diseases, while Leishmania tarentolae infects amphibians and became a biotechnological tool suitable for recombinant protein expression. T. cruzi antigens are needed for the development of improved epitope-based methods for diagnosis and treatment of Chagas disease. Molecular cloning for the production of recombinant proteins offers the possibility to obtain T. cruzi antigens at high yield and purity. L. tarentolae appears as the ideal expression host to obtain recombinant T. cruzi antigens with a structure and posttranslational modifications typical of trypanosomatids. In this chapter, we present a protocol for the analytical to mid-scale production of recombinant T. cruzi antigens, using L. tarentolae as expression host (LEXSY ® inducible system).