info:eu-repo/semantics/article
Hair Cortisol Measurement by an Automated Method
Fecha
2019-06Registro en:
Gonzalez, Diego Javier; Jacobsen, Dario Gustavo; Ibar, Carolina; Pavan, Carlos Humberto; Monti, José Luis Eugenio; et al.; Hair Cortisol Measurement by an Automated Method; Nature Publishing Group; Scientific Reports; 9; 1; 6-2019; 1-6
2045-2322
CONICET Digital
CONICET
Autor
Gonzalez, Diego Javier
Jacobsen, Dario Gustavo
Ibar, Carolina
Pavan, Carlos Humberto
Monti, José Luis Eugenio
Fernandez Machulsky, Nahuel Hernan
Balbi, Ayelen
Fritzler, Melisa Araceli
Jamardo, Juan
Repetto, Esteban M.
Berg, Gabriela Alicia
Fabre, Bibiana
Resumen
We present the development of the first procedure for hair cortisol measurement through an automated method. Hair samples were obtained from 286 individuals. After cortisol extraction, samples were measured in a Siemens Immulite 2000 (Gwynedd, UK) automated chemoluminiscent immunoassay analyzer. Normal reference values were obtained from hair cortisol levels measured in 213 healthy individuals with low levels of stress. Hair cortisol concentration median was 55 pg/mg hair (2.5–97.5 percentile (40–128)) in healthy individuals with low levels of stress and 250 pg/mg hair (range 182–520) in stressed individuals. No significant differences were observed in hair cortisol levels between subjects with and without dye (40 (40–107) and 40 (40–155) pg/mg hair, respectively; p = 0.128). The novel procedure presented here shows an adequate analytical performance.