info:eu-repo/semantics/article
Pregnancy and lactation differentially modify the transcriptional regulation of steroidogenic enzymes through DNA methylation mechanisms in the hippocampus of aged rats
Fecha
2016-07Registro en:
Rossetti, María Florencia; Varayoud, Jorgelina Guadalupe; Lazzarino, Gisela Paola; Luque, Enrique Hugo; Ramos, Jorge Guillermo; Pregnancy and lactation differentially modify the transcriptional regulation of steroidogenic enzymes through DNA methylation mechanisms in the hippocampus of aged rats; Elsevier Ireland; Molecular and Cellular Endocrinology; 429; 7-2016; 73-83
0303-7207
CONICET Digital
CONICET
Autor
Rossetti, María Florencia
Varayoud, Jorgelina Guadalupe
Lazzarino, Gisela Paola
Luque, Enrique Hugo
Ramos, Jorge Guillermo
Resumen
In the present study, we examined the mRNA expression and DNA methylation state of steroidogenic enzymes in the hippocampus of young adult (90-days-old) and middle-aged (450-days-old) nulliparous rats, and middle-aged multiparous rats subjected to three pregnancies with and without lactation. Aging decreased the mRNA levels of steroidogenic-related genes, while pregnancy and lactation significantly reduced the effect of aging, maintaining high expression levels of cytochrome P450 side-chain cleavage (P450scc), steroid 5α-reductase-1 (5αR-1), cytochrome P450arom (P450arom) and aldosterone synthase (P450(11β)-2). In addition, pregnancy and lactation diminished the methylation state of the 5αR-1 promoter and increased the transcription of brain-derived neurotrophic factor, synaptophysin and spinophilin. Pregnancy without lactation increased P450scc and 5αR-1 gene expression and decreased the methylation of their promoters. We concluded that the age-related decrease in the mRNA expression of steroidogenic enzymes is differentially attenuated by pregnancy and lactation in the rat hippocampus and that differential methylation mechanisms could be involved.