info:eu-repo/semantics/article
Metalloproteomics analysis in human mammary cell lines treated with inorganic mercury
Fecha
2020-03Registro en:
Ávila Maniero, Mariángeles; Wuilloud, Rodolfo German; Callegari, Eduardo Alberto; Smichowski, Patricia Nora; Fanelli, Mariel Andrea; Metalloproteomics analysis in human mammary cell lines treated with inorganic mercury; Elsevier Gmbh; Journal of Trace Elements in Medicine and Biology; 58; 3-2020
0946-672X
CONICET Digital
CONICET
Autor
Ávila Maniero, Mariángeles
Wuilloud, Rodolfo German
Callegari, Eduardo Alberto
Smichowski, Patricia Nora
Fanelli, Mariel Andrea
Resumen
The interest in inorganic Hg toxicity and carcinogenicity has been pointed to target organs such as kidney, brain or placenta, but only a few studies have focused on the mammary gland. In this work, analytical combination techniques (SDS-PAGE followed by CV-AFS, and nanoUPLC-ESI-MS/MS) were used to determine proteins that could bind Hg in three human mammary cell lines. Two of them were tumorigenic (MCF-7 and MDA-MB-231) and the other one was the non-tumorigenic cell line (MCF-10A). There are no studies that provide this kind of information in breast cell lines with IHg treatment. Previously, we described the viability, uptake and the subcellular distribution of Hg in human breast cells and analysis of RNA-seq about the genes that encode proteins which are related to cytotoxicity of Hg. This work provides important protein candidates for further studies of Hg toxicity in the mammary gland, thus expanding our understanding of how environmental contaminants might affect tumor progression and contribute with future therapeutic methods.