info:eu-repo/semantics/article
Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation
Fecha
2008-03Registro en:
Juri Ayub, Maximiliano; Ma, Kit-Wan; Shaw, Pang-Chui; Wong, Kam-Bo; Trypanosoma cruzi: High ribosomal resistance to trichosanthin inactivation; Academic Press Inc Elsevier Science; Experimental Parasitology; 118; 3; 3-2008; 442-447
0014-4894
CONICET Digital
CONICET
Autor
Juri Ayub, Maximiliano
Ma, Kit-Wan
Shaw, Pang-Chui
Wong, Kam-Bo
Resumen
Trypanosoma cruzi is the parasite causing Chagas Disease. Several results already published suggest that T. cruzi ribosomes have remarkable differences with their mammalian counterparts. In the present work, we showed that trypanosomatid (T. cruzi and Crithidia fasciculata) ribosomes are highly resistant to inactivation by trichosanthin (TCS), which is active against mammalian ribosomes. Differential resistance is an intrinsic feature of the ribosomal particles, as demonstrated by using assays where the only variable was the ribosomes source. Because we have recently described that TCS interacts with the acidic C-terminal end of mammalian ribosomal P proteins, we assayed the effect of a TCS variant, which is unable to interact with P proteins, on trypanosomatid ribosomes. This mutant showed similar shifting of IC50 values on rat, T. cruzi and C. fasciculata ribosomes, suggesting that the resistance mechanism might involve other ribosomal components rather than the C-terminal end of P proteins.