info:eu-repo/semantics/article
Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae
Fecha
2019-12Registro en:
Yauri Condor, Katherine; Gonzales Escalante, Edgar; Di Conza, José Alejandro; Gutkind, Gabriel Osvaldo; Detection of plasmid-mediated colistin resistance by colistin pre-diffusion and inhibition with EDTA test (CPD-E) in Enterobactereaceae; Elsevier Science; Journal of Microbiological Methods; 167; 12-2019; 1-4
0167-7012
CONICET Digital
CONICET
Autor
Yauri Condor, Katherine
Gonzales Escalante, Edgar
Di Conza, José Alejandro
Gutkind, Gabriel Osvaldo
Resumen
A phenotypic assay based on colistin pre-diffusion and differential inhibition of Mobile Colistin Resistance (MCR) protein activity by EDTA showed that, of the 92 strains tested, all MCR producers (49) exhibited an increase ≥5 mm in the inhibition zone around the area of pre-diffusion in the presence of EDTA, in comparison with colistin alone. Results suggest that CPD-E may differentiate MCR-producing microorganisms from resistant microorganisms without this marker.