info:eu-repo/semantics/article
Glyphosate induces epithelial mesenchymal transition-related changes in human endometrial Ishikawa cells via estrogen receptor pathway
Fecha
2020-04Registro en:
Gastiazoro, Maria Paula; Durando, Milena de Lourdes; Milesi, Maria Mercedes; Lorenz, Virginia; Vollmer, G.; et al.; Glyphosate induces epithelial mesenchymal transition-related changes in human endometrial Ishikawa cells via estrogen receptor pathway; Elsevier Ireland; Molecular and Cellular Endocrinology; 510; 4-2020
0303-7207
CONICET Digital
CONICET
Autor
Gastiazoro, Maria Paula
Durando, Milena de Lourdes
Milesi, Maria Mercedes
Lorenz, Virginia
Vollmer, G.
Varayoud, Jorgelina Guadalupe
Zierau, O.
Resumen
Glyphosate based herbicides are the most commonly used herbicide in the world. We aimed to determine whether glyphosate (Gly) induces epithelial mesenchymal transition (EMT) - related changes in a human endometrial carcinoma cell line (Ishikawa cells), and whether the estrogen receptor (ER) pathway is involved inthese changes. Ishikawa cells were exposed to Gly (0.2 μM and 2 μM) or 17β-estradiol (E2: 10−9 M). We detected that Gly increased cell migration and invasion ability compared to vehicle, as did E2. Moreover, a down regulation of E-cadherin mRNA expression was determined in response to Gly, similar to E2-effects. These results show that Gly promotes EMT-related changes in Ishikawa cells. When an ER antagonist (Fulvestrant: 10−7 M) was co-administrated with Gly, all changes were reversed, suggesting that Gly might promote EMT-related changes via ER-dependent pathway. Our results are interesting evidences of Gly effects on endometrial cancer progression via the ER-dependent pathway.