info:eu-repo/semantics/article
Microenvironments to study migration and somal translocation in cortical neurons
Fecha
2018-02Registro en:
Zhao, Shifang; Fan, Wenqiang; Guo, Xiang; Xue, Longjian; Berninger, Benedikt; et al.; Microenvironments to study migration and somal translocation in cortical neurons; Elsevier; Biomaterials; 156; 2-2018; 238-247
0142-9612
CONICET Digital
CONICET
Autor
Zhao, Shifang
Fan, Wenqiang
Guo, Xiang
Xue, Longjian
Berninger, Benedikt
Salierno, Marcelo Javier
del Campo, Aránzazu
Resumen
Migrating post-mitotic neurons of the developing cerebral cortex undergo terminal somal translocation (ST) when they reach their final destination in the cortical plate. This process is crucial for proper cortical layering and its perturbation can lead to brain dysfunction. Here we present a reductionist biomaterials platform that faithfully supports and controls the distinct phases of terminal ST in vitro. We developed microenvironments with different adhesive molecules to support neuronal attachment, neurite extension, and migration in distinct manners. Efficient ST occurred when the leading process of migratory neurons crossed from low-to high-adhesive areas on a substrate, promoting spreading of the leading growth cone. Our results indicate that elementary adhesive cell-substrate interactions strongly influence migratory behavior and the final positioning of neurons during their developmental journey. This in vitro model allows advanced experimentation to reveal the microenvironmental requirements underlying cortical layer development and disorders.