info:eu-repo/semantics/article
Identification of potent bovine viral diarrhea virus inhibitors by a structure-based virtual screening approach
Fecha
2019-01Registro en:
Castro, Eliana Florencia; Casal, Juan José; España de Marco, Maria Jose; Battini, Leandro; Fabiani, Matias; et al.; Identification of potent bovine viral diarrhea virus inhibitors by a structure-based virtual screening approach; Elsevier; Biorganic and Medicinal Chemistry Letters; 29; 2; 1-2019; 262-266
0960-894X
CONICET Digital
CONICET
Autor
Castro, Eliana Florencia
Casal, Juan José
España de Marco, Maria Jose
Battini, Leandro
Fabiani, Matias
Fernandez, Gabriela Araceli
Bruno, Ana María
Cavallaro, Lucia Vicenta
Bollini, Mariela
Resumen
Bovine viral diarrhea virus (BVDV) is a pestivirus whose infection in cattle is globally distributed. The use of antivirals could complement vaccination as a tool of control and reduce economic losses. The RNA-dependent RNA polymerase (RdRp) of the virus is essential for its genome replication and constitutes an attractive target for the identification of antivirals. With the aim of obtaining selective BVDV inhibitors, the crystal structure of BVDV RdRp was used to perform a virtual screening. Approximately 15,000 small molecules from commercial and in-house databases were evaluated and several structurally different compounds were tested in vitro for antiviral activity. Interestingly, of twelve evaluated compounds, five were active and displayed EC 50 values in the sub and low-micromolar range. Time of drug addition experiment and measured intracellular BVDV RNA showed that compound 7 act during RNA synthesis. Molecular Dynamics and MM/PBSA calculation were done to characterize the interaction of the most active compounds with RdRp, which will allow future ligand optimization. These studies highlight the use of in silico screening to identify a new class of BVDV inhibitors.