info:eu-repo/semantics/article
Development and evaluation of novel nanophotosensitizers as photoantimicrobial agents against Staphylococcus aureus
Fecha
2019-01-01Registro en:
Gualdesi, María Soledad; Aiassa, Virginia; Vara, Jimena; Alvarez Igarzabal, Cecilia Ines; Ortiz, Cristina Susana; Development and evaluation of novel nanophotosensitizers as photoantimicrobial agents against Staphylococcus aureus; Elsevier Science; Materials Science and Engineering: C; 94; 1-1-2019; 303-309
0928-4931
CONICET Digital
CONICET
Autor
Gualdesi, María Soledad
Aiassa, Virginia
Vara, Jimena
Alvarez Igarzabal, Cecilia Ines
Ortiz, Cristina Susana
Resumen
The main aim of the present study was to synthetize polyacrylamide nanoparticles and to use them as photosensitizer carriers. The new monobrominated derivatives (monobrominated neutral red and monobrominated azure B) were the photosensitizers used for antimicrobial photodynamic therapy. They were loaded into the nanocarriers and their antibacterial and oxidative activities were evaluated. The polyacrylamide nanoparticles were evaluated and prepared by inverse microemulsion polymerization. The nanoparticles obtained were characterized by size, polydispersity index, and zeta potential analysis. The Dynamic Light Scattering indicated that the diameter of the particle (z-average) was optimal, with an acceptable polydispersity index. The antibacterial activity of the polyacrylamide nanoparticles loaded with photosensitizers was evaluated against Staphylococcus aureus. Both photosensitizers loaded into the nanoparticles showed great potential as antibacterial agents since they suppressed the bacterial growth. The maximum percentage of growth reduction was 35.5% (>2 Log CFU/mL), with the monobrominated azure B loaded into the nanocarrier with 2 hydroxyethyl methacrylate against methicillin resistant S. aureus. The improved physicochemical and photophysical properties of these photosensitizers were accompanied by a significant increase in the photoantimicrobial action, in conventional-sensitive and-methicillin resistant S. aureus. The results obtained clearly suggest that polyacrylamide nanoparticles loaded with photosensitizers have great potential for further application in antimicrobial photodynamic therapy.