info:eu-repo/semantics/article
Linear polyethylenimine-decorated gold nanoparticles: One-step electrodeposition and studies of interaction with viral and animal proteins
Fecha
2019-04Registro en:
Lazaro Martinez, Juan Manuel; Byrne, Agustin Jesus; Rodríguez Castellón, Enrique; Manrique, Julieta Marina; Jones, Leandro Roberto; et al.; Linear polyethylenimine-decorated gold nanoparticles: One-step electrodeposition and studies of interaction with viral and animal proteins; Pergamon-Elsevier Science Ltd; Electrochimica Acta; 301; 4-2019; 126-135
0013-4686
CONICET Digital
CONICET
Autor
Lazaro Martinez, Juan Manuel
Byrne, Agustin Jesus
Rodríguez Castellón, Enrique
Manrique, Julieta Marina
Jones, Leandro Roberto
Campodallorto, Viviana
Resumen
Polyethylenimine (PEI)-decorated gold nanoparticles (AuNP) were electrodeposited on conductive surfaces in one-step procedure. Solution-state NMR evidenced that chloride from PEI.HCl was partially exchanged by AuCl 4 − prior to AuNP formation. XPS studies indicated the presence of Au° together with the interaction between AuNP with nitrogen atoms of the PEI polymer. The particle size by DLS was 49 nm. The electrochemical behavior of bare electrode and of glassy carbon electrode modified with PEI-decorated AuNP was compared using [Fe(CN) 6 ] 3-/4- redox probe, to determine the potential role of the nanomaterial and of the polymer in the detection of proteins. The most relevant experimental variables from cyclic voltammetry (CV), square-wave voltammetry (SWV) and electrochemical impedance spectroscopy (EIS) were used for the characterization of protein uptake. The adsorption by electrostatic interaction between the biomolecules and the positively charged polymer (PEIH + ) affected negatively the current response (Ip) of the probe, especially when the negatively charged protein was involved. This platform resulted adequate to immobilize proteins and to characterize this process, for further applications as a tool in bioanalysis or biotechnology.