Protocol for evaluating neuronal polarity in murine models
Wilson Rodriguez, Carlos; Rozés Salvador, María Victoria; Martínez Cáceres, Alfredo Iván; Protocol for evaluating neuronal polarity in murine models; Cell Press; STAR Protocols; 1; 3; 12-2020; 1-20
Wilson Rodriguez, Carlos
Rozés Salvador, María Victoria
Martínez Cáceres, Alfredo Iván
The establishment of polarity is crucial for the physiology and wiring of neurons. Therefore, monitoring the axo-dendritic specification allows the mechanisms and signals associated with development, growth, and disease to be explored. Here, we describe major and minor steps to study polarity acquisition, using primary cultures of hippocampal neurons isolated from embryonic rat hippocampi, for in vitro monitoring. Furthermore, we use in utero electroporated, GFP-expressing embryonic mouse brains for visualizing cortical neuron migration and polarization in situ. Some underreported after-protocol steps are also included. For complete details on the use and execution of this protocol, please refer to Wilson et al. (2020).