info:eu-repo/semantics/article
Antiproliferative effect of amaranth proteins and peptides on HT-29 human colon tumor cell line
Fecha
2019-01Registro en:
Sabbione, Ana Clara; Ogutu, Fredrick Oñandu; Scilingo, Adriana Alicia; Zhang, Miao; Añon, Maria Cristina; et al.; Antiproliferative effect of amaranth proteins and peptides on HT-29 human colon tumor cell line; Springer; Plant Foods for Human Nutrition; 74; 1-2019; 107-114
0921-9668
1573-9104
CONICET Digital
CONICET
Autor
Sabbione, Ana Clara
Ogutu, Fredrick Oñandu
Scilingo, Adriana Alicia
Zhang, Miao
Añon, Maria Cristina
Mu, Tai-Hua
Resumen
Antiproliferative effect of Amaranthus mantegazzianus proteins and peptides released after simulated gastrointestinal digestion (DH% 37.8 ± 3.8) was investigated on human colon cancer cell line (HT-29). Inhibition of proliferation of HT-29 cells was exhibited after a 24 h treatment with different concentrations of amaranth protein isolate (API) and the peptides released after digestion (DGS), presenting IC50 values of 1.35 ± 0.12 and 0.30 ± 0.07 mg soluble protein/mL, respectively. Lactate dehydrogenase assay indicated that both samples caused the loss of membrane integrity and cell lysis over HT-29 cells, and DAPI fluorescence microscopies evidenced typical apoptotic features. Moreover, Annexin V-FITC flow cytometry showed a significant increase of early apoptotic and late apoptotic/necrotic HT-29 cells compared to untreated ones, and caspase-3 assay confirmed the apoptosis induction with a 43.0 ± 10.3% and 65.8 ± 12.7% increase of caspase-3 activity produced by a 2 mg/mL treatment of API and DGS, respectively. In conclusion, amaranth peptides successfully released after simulated gastrointestinal digestion would exert a potential antiproliferative activity over HT-29 tumor cells. This effect was linked to the induction of cell necrosis and apoptosis, supporting the idea of using amaranth proteins as a potential food alternative ingredient for functional foods.