info:eu-repo/semantics/article
Chitosan and cloxacillin combination improve antibiotic efficacy against different lifestyle of coagulase-negative Staphylococcus isolates from chronic bovine mastitis
Fecha
2018-12-23Registro en:
Breser, Maria Laura; Felipe, Verónica; Bohl, Luciana Paola; Orellano, María Soledad; Isaac, Paula; et al.; Chitosan and cloxacillin combination improve antibiotic efficacy against different lifestyle of coagulase-negative Staphylococcus isolates from chronic bovine mastitis; Nature Publishing Group; Scientific Reports; 8; 1; 23-12-2018
2045-2322
CONICET Digital
CONICET
Autor
Breser, Maria Laura
Felipe, Verónica
Bohl, Luciana Paola
Orellano, María Soledad
Isaac, Paula
Conesa, Agustin
Rivero, Virginia Elena
Correa, Silvia Graciela
Bianco, Ismael Dario
Porporatto, Carina
Resumen
Bovine mastitis affects the health of dairy cows and the profitability of herds worldwide. Coagulase-negative staphylococci (CNS) are the most frequently isolated pathogens in bovine intramammary infection. Based on the wide range of antimicrobial, mucoadhesive and immunostimulant properties demonstrated by chitosan, we have evaluated therapy efficiency of chitosan incorporation to cloxacillin antibiotic as well as its effect against different bacterial lifestyles of seven CNS isolates from chronic intramammary infections. The therapeutic effects of combinations were evaluated on planktonic cultures, bacterial biofilms and intracellular growth in mammary epithelial cells. We found that biofilms and intracellular growth forms offered a strong protection against antibiotic therapy. On the other hand, we found that chitosan addition to cloxacillin efficiently reduced the antibiotic concentration necessary for bacterial killing in different lifestyle. Remarkably, the combined treatment was not only able to inhibit bacterial biofilm establishment and increase preformed biofilm eradication, but it also reduced intracellular bacterial viability while it increased IL-6 secretion by infected epithelial cells. These findings provide a new approach to prophylactic drying therapy that could help to improve conventional antimicrobial treatment against different forms of bacterial growth in an efficient, safer and greener manner reducing multiresistant bacteria generation and spread.