Argentina
| info:eu-repo/semantics/article
Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages
Registro en:
Briggiler Marcó, Mariángeles; de Antoni, Graciela Liliana; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Lujan; Thermal, chemical and photocatalytic inactivation of Lactobacillus plantarum bacteriophages; International Association for Food Protection; Journal of Food Protection; 72; 5; 5-2009; 1012-1019
0362-028X
CONICET Digital
CONICET
Autor
Briggiler Marcó, Mariángeles
de Antoni, Graciela Liliana
Reinheimer, Jorge Alberto
Quiberoni, Andrea del Lujan
Resumen
The effect of several biocides, thermal treatments, and photocatalysis on the viability of four Lactobacillus plantarum phages was investigated. Times to achieve 99% inactivation (T99) of phages at 63, 72, and 90°C were evaluated in four suspension media: deMan Rogosa Sharpe broth, reconstituted skim milk, a commercial EM-glucose medium, and Tris magnesium gelatin buffer. The four phages studied were highly resistant to 63°C (T99 > 45 min); however, counts < 10 PFU/ml were achieved by heating at 90°C for 5 min. Higher thermal resistance at 72°C was observed when reconstituted skim milk and EM-glucose medium were assayed. Peracetic acid (0.15%, vol/vol) was an effective biocide for the complete inactivation of all phages studied within 5 min of exposure. Sodium hypochlorite (800 ppm) inactivated the phages completely within 30 min. Ethanol (100%) did not destroy phage particles even after 45 min. Isopropanol did not have any effect on phage viability. Phage counts < 50 PFU/ml were obtained within 180 min of photocatalytic treatment. The results obtained in this work are important for establishing adequate methods for inactivating phages in industrial plants and laboratory environments. Fil: Briggiler Marcó, Mariángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: de Antoni, Graciela Liliana. Universidad Nacional de La Plata; Argentina Fil: Reinheimer, Jorge Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Quiberoni, Andrea del Lujan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Lactología Industrial. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina