Determination of progesterone (P4) from bovine serum samples using a microfluidic immunosensor system

dc.creatorArevalo, Fernando Javier
dc.creatorMessina, Germán Alejandro
dc.creatorMolina, Patricia Gabriela
dc.creatorZon, María Alicia
dc.creatorRaba, Julio
dc.creatorFernández, Héctor
dc.date2010-03
dc.identifierhttp://hdl.handle.net/11336/92850
dc.identifierArevalo, Fernando Javier; Messina, Germán Alejandro; Molina, Patricia Gabriela; Zon, María Alicia; Raba, Julio; et al.; Determination of progesterone (P4) from bovine serum samples using a microfluidic immunosensor system; Elsevier Science; Talanta; 80; 5; 3-2010; 1986-1992
dc.identifier0039-9140
dc.identifierCONICET Digital
dc.identifierCONICET
dc.descriptionProgesterone (P4) is a steroidal hormone with a vital role in the maintenance of human and animal health. This paper describes the development of an immunosensor coupled to glassy carbon (GC) electrode and integrated to a microfluidic system to quantify P4 from bovine serum samples in a fast and sensitive way. The serum samples spiked with a given P4 concentration and a given P4 concentration bound to horseradish peroxide (HPR) were simultaneously added and, therefore, they competed immunologically with sheep monoclonal anti-P4 antibodies that were immobilized at a rotating disk. HRP in the presence of hydrogen peroxide (H2O2) catalyzes the chatecol (H2Q) oxidation to benzoquinone (Q). Its reverse electrochemical reduction to H2Q can be detected at a GC electrode surface at -0.15 V by chronoamperometric measurements. These current responses are proportional to the enzyme activity and inversely proportional to the P4 amount present in bovine serum samples. This P4 immunosensor showed a linear working range from 0.5 to 12.5 ng mL-1. The detection (DL) and quantification (QL) limits were 0.2 and 0.5 ng mL-1, respectively. The electrochemical immunosensor had a higher sensitivity than the ELISA method using conventional spectrophotometric detections. However, both methods allowed us to obtain similar detection limits. The immunosensor allowed us to make up to 100 determinations on different samples without any previous pre-treatment. This behavior proved to be suitable to detect P4 in routine veterinary, clinical, biological, physiological, and analytical assays.
dc.descriptionFil: Fernando Javier Arévalo. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales; Argentina
dc.descriptionFil: Messina, Germán Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
dc.descriptionFil: Molina, Patricia Gabriela. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Area Electroquímica; Argentina
dc.descriptionFil: Zon, María Alicia. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Area Electroquímica; Argentina
dc.descriptionFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina; Argentina
dc.descriptionFil: Héctor Fernández. Universidad Nacional de Rio Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química. Area Electroquímica; Argentina
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dc.languageeng
dc.publisherElsevier Science
dc.relationinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.talanta.2009.10.059
dc.relationinfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0039914009008480
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subjectAMPEROMETRIC IMMUNOSENSOR
dc.subjectENZYME IMMUNOASSAYS
dc.subjectFLOW INJECTION ANALYSIS
dc.subjectGLASSY CARBON ELECTRODE
dc.subjectHORSERADISH PEROXIDASE
dc.subjectPROGESTERONE
dc.subjecthttps://purl.org/becyt/ford/1.4
dc.subjecthttps://purl.org/becyt/ford/1
dc.titleDetermination of progesterone (P4) from bovine serum samples using a microfluidic immunosensor system
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:ar-repo/semantics/artículo
dc.typeinfo:eu-repo/semantics/publishedVersion


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