info:eu-repo/semantics/article
Chronic exposure to low concentrations of chlorpyrifos affects normal cyclicity and histology of the uterus in female rats
Fecha
2021-10Registro en:
Ramos Nieto, María Rosa; Lasagna, Marianela; Cao, Gabriel Fernando; Álvarez, Gloria; Santamaría, Clarisa Guillermina; et al.; Chronic exposure to low concentrations of chlorpyrifos affects normal cyclicity and histology of the uterus in female rats; Elsevier Science; Food and Chemical Toxicology; 156; 10-2021; 112515-112515
0278-6915
CONICET Digital
CONICET
Autor
Ramos Nieto, María Rosa
Lasagna, Marianela
Cao, Gabriel Fernando
Álvarez, Gloria
Santamaría, Clarisa Guillermina
Rodriguez Girault, María Eugenia
Bourguignon, Nadia
Di Giorgio, Noelia Paula
Ventura, Clara
Mardirosian, Mariana Noelia
Rodriguez, Horacio Adolfo
Lux, Victoria Adela R.
Cocca, Claudia Marcela
Núñez, Mariel
Resumen
Chlorpyrifos (CPF), the most used insecticide in Argentina, can act as an endocrine disruptor at low doses. We previously demonstrated that chronic exposure to CPF induces hormonal imbalance in vivo. The aim of this work was to study the effects of low concentrations of CPF (0.01 and 1 mg/kg/day) on the reproductive system of virgin adult rats. In the ovary, we studied the effects of CPF on steroidogenesis by determining steroid hormone content by RIA and CYP11 and CYP19 enzyme expression by qRT-PCR. The estrous cycle was evaluated by microscopic observation of vaginal smear, as well as by changes in uterine histology. In endometrium, we determined the fractal dimension and expression of PCNA, ERα and PR by IHC. Our results showed that chronic exposure to CPF affects ovarian steroid synthesis, causing alterations in the normal cyclicity of animals. In addition, CPF induced proliferative changes in the uterus, suggesting that it could affect reproduction or act as a risk factor in the development of uterine proliferative pathologies.