The accumulation of flavonoids was evaluated in organogenic cultures of Alpinia purpurata through HPLC analysis. Cultures were maintained in liquid MS medium in the following treatments: MS0 (control), TDZ, BAP and IAA + TDZ. Kaempferol-3-O-glucuronide content was higher than rutin for all in vitro treatments, except by control medium in which plants did not produce kaempferol-3-O-glucuronide. A remarkable increase in kaempferol-3-O-glucuronide content was verified using BAP 2 mg.l^-1 (0.027 mg/100 mg dried extract) and IAA 2 mg.l^-1 + TDZ 2 mg.l^-1 (0.030 mg/100 mg dried extract) treatments. With the addition of 2 mg.l^-1 BAP, rutin concentration also increased in the proportion 1:4 compared to control.