dc.creatorPATRICIA CANO SANCHEZ
dc.creatorALFREDO TORRES LARIOS
dc.creatorSAMUEL LARA GONZALEZ
dc.creatorEnrique Ortega
dc.creatorAdela Rodriguez-Romero
dc.date2016-09
dc.date.accessioned2022-10-12T19:39:05Z
dc.date.available2022-10-12T19:39:05Z
dc.identifierhttp://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1436
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4119376
dc.description"Oligomerization of allergens plays an important role in IgE-mediated reactions, as effective crosslinking of IgE-Fc epsilon RI complexes on the cell membrane is dependent on the number of exposed B-cell epitopes in a single allergen molecule or on the occurrence of identical epitopes in a symmetrical arrangement. Few studies have attempted to experimentally demonstrate the connection between allergen dimerization and the ability to trigger allergic reactions. Here we studied plant allergenic profilins rHev b 8 (rubber tree) and rZea m 12 (maize) because they represent an important example of cross-reactivity in the latex-pollen-food syndrome. Both allergens in their monomeric and dimeric states were isolated and characterized by exclusion chromatography and mass spectrometry and were used in immunological in vitro experiments. Their crystal structures were solved, and for Hev b 8 a disulfide-linked homodimer was found. Comparing the structures we established that the longest loop is relevant for recognition by IgE antibodies, whereas the conserved regions are important for cross-reactivity. We produced a novel monoclonal murine IgE (mAb 2F5), specific for rHev b 8, which was useful to provide evidence that profilin dimerization considerably increases the IgE-mediated degranulation in rat basophilic leukemia cells."
dc.formatapplication/pdf
dc.publisherNature Publishing Group
dc.relationinfo:eu-repo/semantics/altIdentifier/DOI/https://doi.org/10.1038/srep32552
dc.relationcitation:Mares-Mejía, I. et al. Structural insights into the IgE mediated responses induced by the allergens Hev b 8 and Zea m 12 in their dimeric forms. Sci. Rep. 6, 32552; doi: 10.1038/srep32552 (2016).
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0
dc.subjectinfo:eu-repo/classification/Autor/Immunology
dc.subjectinfo:eu-repo/classification/Autor/X-ray crystallography
dc.subjectinfo:eu-repo/classification/cti/7
dc.subjectinfo:eu-repo/classification/cti/33
dc.titleStructural insights into the IgE mediated responses induced by the allergens Hev b 8 and Zea m 12 in their dimeric forms
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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