Tesis
Identificação de transcritos diferencialmente expressos por Cryptococcus gattii em condições de privação de ferro, caracterização molecular e perfil de susceptibilidade a antifúngicos de isolados clínicos veterinários
Fecha
2014-03-13Registro en:
PAULA, Daphine Ariadne Jesus de. Identificação de transcritos diferencialmente expressos por Cryptococcus gattii em condições de privação de ferro, caracterização molecular e perfil de susceptibilidade a antifúngicos de isolados clínicos veterinários. 2014. 121 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Mato Grosso, Faculdade de Medicina, Cuiabá, 2014.
Autor
Dutra, Valéria
Nakazato, Luciano
http://lattes.cnpq.br/3898850578198054
http://lattes.cnpq.br/4478191386305454
Dutra, Valéria
501.674.720-20
http://lattes.cnpq.br/4478191386305454
Nakazato, Luciano
638.389.071-91
http://lattes.cnpq.br/3898850578198054
501.674.720-20
638.389.071-91
Hahn, Rosane Christine
783.744.807-34
http://lattes.cnpq.br/5133908054482037
Slhessarenko, Renata Dezengrini
006.990.439-13
http://lattes.cnpq.br/3790088995387532
Watanabe, Michelle Igarashi
029.630.899-45
http://lattes.cnpq.br/4783032223702854
Botton, Sônia de Ávila
672.074.720-72
http://lattes.cnpq.br/0814772095155945
Institución
Resumen
Cryptococcus neoformans/gattii complex is a pathogen relevant to public health in Brazil
and other countries. Molecular studies are important to understanding the epidemiology and
biology of this yeast. Deprivation of some micronutrients, among them iron, can be related to
expression of genes that contribute to the pathogenicity of the microorganism. The first part of
this study aimed to analyze transcripts differentially expressed by C. gattii during iron
deprivation using RDA approach (Representational Difference Analysis). Regarding the RDA,
C. gattii expressed genes related to different biological processes such as ergosterol metabolism,
cell cycle, lipid metabolism, transcriptional activators, organization of the wall and cell
membrane. Thus, the second part of this study was to characterize the molecular type of 04 C.
gattii clinical isolates from two cities of Mato Grosso State, determining mating-types, genetic
diversity by PCR-fingerprinting and susceptibility to five antifungals by EtestTM. The four
isolates in this study were identified as C. gattii VGII, MATα. The fungal strains were
susceptible to ketoconazole, fluconazole, amphotericin B, voriconazole and susceptible dosedependent to itraconazole. Our results revealed novel genes involved in iron metabolism and will
contribute to future eco-epidemiological studies in the country.