Tesis
Estudo genético-clínico da displasia coxofemoral em cães shih tzu através de polimorfismos de nucleotídeos simples (SNPs)
Fecha
2018-07-13Registro en:
SÔNEGO, Dábila Araújo. Estudo genético-clínico da displasia coxofemoral em cães shih tzu através de polimorfismos de nucleotídeos simples (SNPs). 2018. 37 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2018.
Autor
Souza, Roberto Lopes de
Nakazato, Luciano
http://lattes.cnpq.br/3898850578198054
http://lattes.cnpq.br/7447876802000519
Souza, Roberto Lopes de
636.664.720-87
http://lattes.cnpq.br/7447876802000519
Galera, Bianca Borsatto
133.329.958-39
http://lattes.cnpq.br/1913176046267188
636.664.720-87
638.389.071-91
Sá, Marcelo Jorge Cavalcanti de
415.649.104-82
http://lattes.cnpq.br/2860610040748121
Dutra, Valéria
501.674.720-20
http://lattes.cnpq.br/4478191386305454
Institución
Resumen
The objective of the study was to verify the incidence of coxofemoral dysplasia (DCF)
in Shih Tzu dogs by radiographic examination and genetic research using three simple
nucleotide polymorphisms (SNPs) already associated with disease in other races. In
all 36 dogs of the Shih Tzu breed were included and submitted to ventrodorsal pelvic
radiography under anesthesia. The images were analyzed by three evaluators,
independently, for classification according to the Organization for For Animals (OFA)
and International Cynological Federation (FCI) regarding the presence or absence of
DCF and its degree. From all animals, whole blood EDTA samples were collected,
from which DNA extraction, polymerase chain reaction (PCR) amplification and
sequencing of the regions corresponding to SNPs BICF2G630227898,
BICF2G630339806 and BICF2S2459425 were performed. Radiographic changes,
articular incongruity, flattening of the femoral head and acetabular deviation were the
most frequent alterations, in 97%, 97% and 91% of the evaluated population,
respectively. According to the severity of these radiographic findings, 61% of the dogs
were classified according to OFA with DCF, while 28% according to the FCI scale.
Referring to the genetic analysis, all sequenced BICF2G630227898 SNPs, 86% SNP
BICF2G630339806 and 27% SNP BICF2S2459425. A second SNP was identified in
the sequenced region near BICF2S2459425 in 62% of the sequenced. The
morphological difference of the involvement in relation to large races to the
radiographic examination, referred to the occurrence of a different disease in Shih Tzu,
but the presence of the SNPs indicates to be the same pathogenesis influenced by
different biomechanical factors, to conclusion, the occurrence must be evaluated in a
broader population.