Tesis
Estudo bioquímico e reprodutivo em ratas wistar prenhes imunossuprimidas pela ciclosporina A
Fecha
2015-02-20Registro en:
LISBOA, Elisangela Miranda de Jesus. Estudo bioquímico e reprodutivo em ratas wistar prenhes imunossuprimidas pela ciclosporina A. 2015. 43 f. Dissertação (Mestrado em Imunologia e Parasitologia Básicas e Aplicadas) - Universidade Federal de Mato Grosso, Instituto de Ciências Biológicas e da Saúde, Barra do Garças, 2015.
Autor
Campos, Kleber Eduardo de
Souto, Paula Cristina de Souza
http://lattes.cnpq.br/2161765071682282
http://lattes.cnpq.br/6939343303863367
Campos, Kleber Eduardo de
270.119.548-99
http://lattes.cnpq.br/6939343303863367
Volpato, Gustavo Tadeu
176.091.941-15
http://lattes.cnpq.br/8276825973471698
270.119.548-99
.
Cora, Luciana Aparecida
259.657.108-01
http://lattes.cnpq.br/9649740010202242
Institución
Resumen
Pregnancy is characterized by complex and intense adaptive physiological changes necessary
for the biological stability for preservation of maternal and fetal health. During pregnancy a
woman may have the need to use drugs, whether for reasons of pre-existing disease or current
pathological condition. However, the use of drugs are able to cross the placenta and reach the
fetus as well, which implies a potential risk for the same. Among the drugs that cross the
placental barrier are immunosuppressive agents which are used for post-transplant women and
in those autoimmune diseases. The objective of this study was to evaluate the effects of
immunosuppressive therapy with Cyclosporine A in reproductive profile and biochemical in
pregnant Wistar rats. The rats were randomized into three groups CONT (treated with tap
water), CLP1 (treated with Cyclosporin A 15 mg / kg before and during pregnancy) and CLP2
(treated with Cyclosporin A 15 mg / kg before pregnancy). The body weight, food and water
intake, and glucose were measured weekly. On the 17th day of pregnancy it was evaluated the
oral glucose tolerance test (OGTT). The reproductive parameters were determined by fertility
indices, pregnancy and childbirth. On the last day of pregnancy (day 21), serum biochemical
analysis were performed (total protein, cholesterol, triglycerides from high density lipoprotein
(HDL) cholesterol and very low density lipoprotein (VLDL). TNF-α levels were determined
serum and placental by enzyme immunoassay. The statistical significance was considered
p<0.05. It was observed a progressive increase in body weight during the pregnancy in all
groups compared to the first day. In addition, the continued use of this drug decreased food
intake and slightly blood glucose. the reproductive parameters have not changed. The
glycemic curve generated by OGTT show the timepoint of 120 minutes of CLP1 group was
increased related to other groups. In biochemical evaluation, TNF-α levels, cholesterol and
HDL were unchanged, but the treatment with the drug led increase in protein concentration
(CLP2), triglyceride and VLDL (CLP1) and also an increase in ALT activity, AST and
reduced (in both groups). We can conclude that the use of Cyclosporine A prior and/or during
pregnancy has some security for weight gain, lipid metabolism and also maternal insulin
action in maternal organism.