Tesis
Detecção e caracterização do Parvovírus canino na região metropolitana de Cuiabá, Mato Grosso, Brasil
Fecha
2012-02-28Registro en:
FONTANA, Danúbia de Souza. Detecção e caracterização do Parvovírus canino na região metropolitana de Cuiabá, Mato Grosso, Brasil. 2012. 59 f. Dissertação (Mestrado em Ciências Veterinárias) - Universidade Federal de Mato Grosso, Faculdade de Agronomia, Medicina Veterinária e Zootecnia, Cuiabá, 2012.
Autor
Pescador, Caroline Argenta
Aguiar, Daniel Moura de
http://lattes.cnpq.br/8668503323187464
http://lattes.cnpq.br/5754349416478829
Pescador, Caroline Argenta
958.659.180-87
http://lattes.cnpq.br/5754349416478829
Aguiar, Daniel Moura de
603.780.181-91
http://lattes.cnpq.br/8668503323187464
958.659.180-87
603.780.181-91
Sousa, Valéria Régia Franco
588.319.911-91
http://lattes.cnpq.br/7722685703977701
Sonne, Luciana
955.938.880-00
http://lattes.cnpq.br/4082158326480104
Institución
Resumen
The canine parvovirus type 2 (CPV-2) is considered a major cause of infectious
diarrhea in dogs less than six months of age. After its emergence in the late 1970s,
CPV-2 spread rapidly in across continents, suffering continuous genetic changes that
gave rise to new antigenic variants (CPV-2a/b/c). The objective of this study was to
detect and genotype CPV-2 by PCR in 50 dogs with clinical, macroscopic and
microscopic lesions suggestive of canine parvovirus, sent to the Veterinary Pathology
Laboratory UFMT for necropsy. Tissue and fecal samples of these dogs were
collected and analyzed by histology and PCR, respectively. Twenty-seven (54%) out
of 50 fecal samples collected had specific DNA of CPV-2. Hemorrhagic diarrhea and
vomiting were the predominant clinical signs. Histologically, hyperemia and serous
rough aspect of the small intestine were the major findings observed, which
corresponded microscopically to extensive areas of necrosis and atrophy of intestinal
villi. Of the 27 positive samples, 13 (48.1%) were randomly selected and typed by
DNA sequence analysis, such as CPV-2c has low genetic variability which some
amino acid changes have not yet observed in previous studies. The findings indicate
that CPV-2c is the main agent for severe CPV-2 enteritis in dogs necropsied at LPVUFMT.