Dissertação
Efeitos do exercício resistido sobre a atividade das enzimas acetilcolinesterase e Na+/K+ ATPase e estresse oxidativo na hipertensão induzida por L-NAME
Fecha
2019-08-26Autor
Lopes, Thauan Faccin
Institución
Resumen
Hypertension (HT) is a disease that affects billions of people around the world, being a
risk factor for numerous other cardiovascular diseases, and may even affect the brain
through poor blood distribution. In experimental models, induced hypertension is by
daily administration of L-NAME. Resistance training has increasingly appeared as an
auxiliary alternative in the treatment of hypertension due to its antihypertensive,
antioxidant and neuroprotective effects. Thus, the objective of this study was to evaluate
the effect of 4 weeks of resistance exercise on enzymatic activities and oxidative stress
parameters in the cerebral cortex of rats with L-NAME-induced hypertension. For this
purpose, 40 rats were divided into four groups: Sedentary Control (Sed-Ctrl), Exercise
Control (Ex-Ctrl), Sedentary L-NAME (Sed-L-NAME) and Exercise L-NAME (Ex-LNAME).
The L-NAME dose used was 30mg / kg / day and began one week before the
exercise protocol. After 48 hours of the last exercise session, the samples were
collected for the evaluations of nitrite and nitrate levels (NOx), total reactive species of
oxygen (tROS), carbonyl protein, reactive species of thiobarbituric acid (TBARS), total
thiols content and the activity of glutathione’s peroxidase (GPx), redutase (GR) and stransferase
(GST), acetilcolinesterase (AChE) and Na+/K+ ATPase. In this experiment,
we saw an L-NAME-induced lower gain of weight, which was restored to normal levels
by the exercise, in the last week. We found increased levels of tROS and thiols in Sed-
L-NAME group, both reverted to standard in Ex-L-NAME group. The NOx content in the
Sed-L-NAME group decreased and the exercise restored it in Ex-L-NAME group. An
increased activity of GPx for the Sed-L-NAME group was observed and its
reestablishment in the Ex-L-NAME group was seen. We also could see an increased
activity in AChE and a reduction in Na+/K+ ATPase of Sed-L-NAME groups, but the
resistance exercise protocol was not able to restore this parameters. The other
measures –TBARS, protein carbonylation, GR and GST- did not present a significant
difference. These results are expected to better understand the damage caused by
hypertension in the central nervous system as well as the effects of this exercise
protocol as a possible aid in the treatment of HT.