Dissertação
Análise fitoquímica e atividade farmacológica in vitro e in vivo de Schinus molle L.
Fecha
2019-08-27Autor
Lovatto, Marina Ortiz
Institución
Resumen
The species S. molle L. is widely distributed in Rio Grande do Sul. The aerial
parts of S. molle are popularly used for different therapeutic purposes as antiinflammatory,
anti-haemorrhagic, antiseptic, mild laxative, astringent, anti-asthmatic,
digestive stimulant, menstrual, diuretic and tonic disorder. The need to seek new
therapeutic alternatives for respiratory diseases, preferably with minimal side effects,
justifies this work, both from the point of view of scientific and social interest. The
present work aimed to obtain the crude extract of S. molle leaves by the maceration
process, perform phytochemical analysis, identify and quantify compounds present in
the extract, evaluate biological activities, such as anti-inflammatory and antimicrobial
activity, as well as evaluate cyto and genotoxicity and antioxidant potential against
reactive oxygen and nitrogen species. S. molle extract after analysis showed
concentrations of 79,14; 231,67; 52,65 mg equivalents/g of extract that were
determined by phytochemical dosages in S. molle extract for total phenols, tannins
and flavonoids, respectively. S. molle was able to capture reactive oxygen and
nitrogen species with antioxidant capacity with IC50 = 30.54 ± 0.41 μg/mL. It did not
present cytotoxicity and genotoxicity, being a safety indicator. Antimicrobial activity
was not significant in relation to the microorganisms Klebsiella pneumoniae,
Escherichia coli, Pseudomonas aeruginosa and Mycobaterium tuberculosis, M.
abcessus, M. massiliense, M. fortuitum and M. smegmatis. The extract showed no
hematological alterations, even in the group induced pleurisy with 2% carrageenan.
The flavonoids rutin (58,63 mg/g) and quercetin (0,665 mg/g) were identified and
quantified.