Dissertação
Óleo essencial de aloysia triphylla em juvenis de jundiá (Rhamdia quelen) intoxicados com ocratoxina A
Fecha
2021-04-09Autor
Camargo, Flávia Constância de Los Santos de
Institución
Resumen
The objective of this study was to analyze the impact of the use of Aloysia triphylla
essential oil (OEAT) at a concentration of 2 mL/Kg, added to the feed of dentex
(Rhamdia quelen) against ochratoxin A intoxication in two concentrations (200 and 400
µg/Kg). Male and female fry (R. quelen) fed diets containing ochratoxin A were used
to evaluate liver and kidney damage and growth parameters. The fish were divided
into six treatments with three repetitions; each treatment was individualized in a closed
recirculation system consisting of 3 boxes, and a total of 18 boxes containing 40L of
water containing seven fish per repetition were used. The treatments were as follows:
Control; OEAT 2 mL/Kg, 200µg ochratoxin + OEAT 2 mL/Kg; 400µg ochratoxin +
OEAT 2 mL/Kg and 200µg ochratoxin and 400µg ochratoxin without the presence of
OEAT. The experiment lasted 32 days and on the last day of the experiment the
animals were weighed, slaughtered and whole blood was collected for processing and
plasma was obtained for plasma analysis of enzymes and metabolites indicators of
kidney and liver damage. The levels of TGO and TGP were evaluated as indicators of
hepatic damage and the treatment with 400 µg/Kg of ochratoxin A without the essential
oil obtained a significant superior difference in relation to the two enzymes, indicating
that the use of the essential oil of A. triphylla in the concentration of 2 mL/Kg protected
the group treated with 400 µg/Kg of ochratoxin A associated with OEAT from hepatic
damage. The creatinine level obtained significant higher difference only in the group
treated with 400µg of OTA+OEAT, demonstrating a possibly synergistic effect. The
total protein value differed statistically lower only in the 2 mL/Kg OEAT group. The
parameters of growth, lactate and mortality were not significantly different. Therefore,
we conclude that the use of OEAT at a concentration of 2 mL/Kg in juvenile dolphins
is beneficial in relation to liver protection caused by ochratoxin A.