Artigo
Dendritic Cells Transfected with scFv from Mab 7.B12 Mimicking Original Antigen gp43 Induces Protection against Experimental Paracoccidioidomycosis
Fecha
2011-01-07Registro en:
Plos One. San Francisco: Public Library Science, v. 6, n. 1, 6 p., 2011.
1932-6203
WOS000286512900013.pdf
10.1371/journal.pone.0015935
WOS:000286512900013
Autor
Ferreira, Karen Spadari [UNIFESP]
Maranhao, Andrea Q.
Garcia, Maria C. C.
Brigido, Marcelo M.
Santos, Suelen S.
Lopes, Jose D. [UNIFESP]
Almeida, Sandro R.
Institución
Resumen
Paracoccidioidomycosis (PCM), endemic in Latin America, is a progressive systemic mycosis caused by Paracoccidioides brasiliensis (P. brasiliensis), which primarily attacks lung tissue. Dendritic cells (DCs) are able to initiate a response in naive T cells, and they also participate in Th-cell education. Furthermore, these cells have been used for therapy in several disease models. Here we transfected DCs with a plasmid (pMAC/PS-scFv) encoding a single chain variable fragment (scFv) of an anti-Id antibody that is capable of mimicking gp43, the main antigenic component of P. brasiliensis. First, Balb/c mice were immunized subcutaneously with pMAC/PS-scFv and, after seven days, scFv protein was presented to the regional lymph nodes cells. Moreover, we showed that the DCs transfected with scFv were capable of efficiently activating proliferation of total lymph node cells and inducing a decrease in lung infection. Therefore, our results suggested that the use of scFv-transfected DCs may be a promising therapy in the paracoccidioidomycosis (PCM) model.