Artigo
An improved method to distinguish Entamoeba histolytica and Entamoeba dispar
Fecha
1999-10-01Registro en:
Parasitology. New York: Cambridge Univ Press, v. 119, p. 359-362, 1999.
0031-1820
10.1017/S0031182099004886
WOS:000083210500005
Autor
Gomes, M. A.
Pesquero, J. B.
Furst, C.
Valle, P. R.
Pesquero, J. L.
Silva, E. F.
Institución
Resumen
A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. the amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. the method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.